專任師資

葉靖輝 博士
葉靖輝 博士 職稱: 助理教授
學歷: 國立台灣大學植物所
專長: 植物逆境生理學、蛋白質體學、植物組織培養、訊息傳遞
信箱: yehch63@cc.ncu.edu.tw
分機: 65068, 65078

個人介紹

  1. 職 稱:助理教授
  2. 學 歷:
    1. 09/1994 ~ 06/1999 Department of Botany, National Taiwan University. PhD Degree.
    2. 09/1983 ~ 06/1987 Department of Botany, National Taiwan University, B.S. Degree.
  3. 經 歷:
    1. 03/2002 ~07/2003 Dr. Tuan-Hua David Ho’s Lab Postdoctoral Research Fellow, Department of Biology, Washington University in St. Louis
    2. 02/2001 ~ 07/2001 Adjunct Assistant Professor, Department of Life Sciences, TzuChi University.
    3. 08/1999 ~ 01/2002 Dr. Chu-Yung Lin’s Lab Postdoctoral Research Fellow, Department of Botany, National Taiwan University.
  4. 現 職: 2003-present 國立中央大學 生命科學研究所 助理教授
  5. 聯絡方式:(03)422-7151 (分機 65068)
  6. FAX:(03) 422-8482
  7. E-mail:yehch63@cc.ncu.edu.tw

授課

  1. 普通生物學
  2. 植物學
  3. 專題討論
  4. 高等植物生理
  5. 專題研究植物生物化學
  6. 植物細胞培養技術
  7. 植物生理逆境
  8. 植物荷爾蒙
  9. 植物生長與發育
  10. 植物的基因轉型作用
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研究領域

植物因無法移動,當遭遇非生物逆境時(如高溫、低溫、乾旱、高塩及重金屬等),常會誘導特別的基因表現以應付此不良環境,但許多相關逆境表現基因的生理功能與作用機制皆尚清楚了解,因此我們實驗室即針對逆境基因進行一系列分析,探討逆境基因的生理功能與調節機制,主要研究方向分成下列三部分:

1.小分子量熱休克蛋白質(Small Heat Shock Proteins; sHSPs)生理功能與調節機制分析:

小分子量熱休克蛋白質普遍存在於原核與真核生物中,它們被視為與生物耐熱性有密切關聯性。我們的研究目標主要針對水稻第一群小分子量熱休蛋白質進行研究,首先探討此群基因的誘導機制,利用基因槍與原生質體方式進行啟動子分析,探討基因啟動子上具有那些特殊元素可接受各種逆境的誘導,目前已分析出一段對Azetidine具反應之元素(Azetidine responsive element;AZRE);接著以生化分析方法探討此群蛋白質的功能區域及蛋白質的結構;最後以基因轉植的方式,藉轉植植物外部型態及生理功能變化瞭解此群基因的生理功能。目標是改善水稻的耐熱性,免於受近來溫室效應的影響。

圖一、水稻第一族小分子量熱休克蛋白質調控機制模式

2.晚胚蛋白質(Late Embryogenesis Abundant Proteins; LEA proteins)生理功能分析:

晚胚蛋白質大量存在於胚胎發育後期,主要功能為增加植物種子耐熱能力,近來動物細胞中亦陸續被發現此類蛋白質的存在。我們研究的目標是水稻第三群的晚胚蛋白質,利用Bioinformatics技術由水稻基因組中篩選出五個水稻第三群的晚胚蛋白質基因-AP004018/BAD19162, AP003381/BAB86507, AC073556/AAL84288, AC098833/AAU43988, AP000836/BAD81113,以短暫轉殖系統與穩定轉殖系統分析它們的表現條件及生理功能;同時分析轉殖植物對不同逆境之耐性,藉此改善水稻抵抗乾燥與其它逆境之能力,增加水稻產量。

圖二、以小角度X光繞射方式分析Oshsp16.9A於高溫下結構之變化

3.蝴蝶蘭開花基因選殖與功能分析:

我國重要花卉-蝴蝶蘭商業栽培皆已低溫處理誘導開花。為了解蝴蝶蘭之開花機制,我們由低溫處理過樣品中建立之基因庫中,篩選出兩個開花相關基因-COL1 & COL2,現即以此兩基因為對象,首先了解它們的生理功能,驗證是否參與調控蝴蝶蘭的開花機制中,接著以基因槍與原生質體方式進行啟動子分析,探討基因啟動子上具有那些特殊元素與基因表現之誘導條件,期能將研究成果應用於調節蝴蝶蘭開花時間之可能性,建立蝴蝶蘭開花的分子機制。

圖三、水稻第三群晚胚蛋白質基因

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論文發表

PAPERS

  1. Guan J-C, Yeh C-H, Lin Y-P, Ke Y-T, Chen M-T, You J-W, Liu Y-H, Lu C-A, Wu S-J, Lin CY*. (2010) A 9bp cis-element in the promoters of class I small heat shock protein genes on chromosome 3 in rice mediates L-azetidine-2-carboxylic acid and heat shock responses. J. Exp. Bot. 2010 Jul 19. [Epub ahead of print]
  2. Wu SJ, Wang LC, Yeh CH, Lu CA, and Wu SJ*. Isolation and characterization of the Arabidopsis heat-intolerant 2 (hit2) mutant reveal the essential role of the nuclear export receptor EXPORTIN1A (XPO1A) in plant heat tolerance. New Phytologist 2010. 186: 833-842. (SCI)
  3. Huang CK, Huang LF, Huang JJ, Wu SJ, Yeh CH, and Lu CA*. A DEAD-box protein, AtRH36, is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis. Plant and Cell Physiology 2010. 51: 694-706. (SCI)
  4. Wang WK, Chia-Chin Liu, Chiang TY, Ming-Tse Chen, Chou CH, and Yeh CH*. Characterization of expressed sequence tags from flower buds of alpine Lilium formosanum using a Subtractive cDNA Library. Plant Molecular Biology Reporter 2010. In press. (SCI)
  5. Wang LC, *Yeh CH, Sayler RJ, Lee YY, Lu CA, and Wu SJ (2008) Arabidopsis HIT1, a putative homolog of yeast tethering protein Vps53p, is required for pollen tube elongation. Botanical bulletin of Academia Sinica 49, 25-32.
  6. *Yeh CH, Wu SJ, Chen HY, and Lin CY(2007) Physiological effects of azetidine on cellular leakage in soybean seedlings. Plant Science 171, 1124-1130.
  7. Lee CF, Pu HY, Wang LC, Sayler RJ, *Yeh CH, and Wu SJ (2006) Mutation in a homolog of yeast Vps53p accounts for the heat and osmotic hypersensitive phenotypes in Arabidopsis hit1-1 mutant. Planta 224, 330-338.
  8. *Yeh CH, Travis D & Ho T.H.D. (2004) Expression of HVA1, a barley group III LEA protein, confers salt resistance in prokaryotic and eukaryotic cells. In preparation.
  9. Guan JC, Jinn TL, *Yeh CH, Feng SP, Chen YM, and Lin CY (2004) Characterization of the genomic structures and selective expression profiles of nine class I small heat shock protein genes clustered on two chromosomes in rice (Oryza sativa L.) Plant Molecular Biology 56, 795-809.
  10. *Yeh CH, Chiu CC, Chen HI, Chen YM & Lin CY (2004) Physiological and biochemical analysis of soybean (Glycine max) small heat shock proteins induced by azetidine-2-carboxylic acid. Submitted to J of Experimental Botany
  11. *Yeh CH, Chen YM & Lin CY (2002) Functional regions of rice heat shock protein, Oshsp16.9, required for conferring thermotolerance in Escherichia coli. Plant Physiol 128, 661-668 (SCI 5.105)
  12. Chiung YM, Lin BL, *Yeh CH & Lin CY (2000) Heat shock protein (hsp 70)-related epitopes are common allergenic determinants for barley and corn antigens. Electrophoresis 21, 297-300 (SCI 4.282).
  13. Young LS, *Yeh CH, Chen YM & Lin CY (1999) Molecular characterization of Oryza sativa 16.9 kDa heat shock protein. Biochemical J. 344, 31-38 (SCI 4.326).
  14. Lin CY, *Yeh CH, Tzeng SS, Tseng TS, Chang FC, Lee YL, Jinn TL & Chang PFL (1997) Class I low-molecualr-weight heat-shock proteins in Oryza sativa. pp41-52. In JC Su ed, Proceedings of Fifth International Symposium on Rice Molecular Biology.
  15. *Yeh CH, Chang PFL, Yeh KW, Lin WC, Chen YM & Lin CY (1997) Expression of a gene encoding a 16.9 kDa heat-shock protein, Oshsp16.9, in Escherichia coli enhances thermotolerance. Proc. Natl. Sci. USA 94: 10967-10972 (SCI 10.896).
  16. *Yeh CH, Yeh KW, Wu SH, Chang PFL, Chen YM & Lin CY (1995) A recombinant rice 16.9-kDa heat shock protein can provide thermotolerance in vitro. Plant Cell Physiol. 36, 1341-1348 (SCI 2.43).
  17. Yeh KW, Jinn TL, *Yeh CH, Chen YM & Lin CY (1994) Plant low-molecular-mass heat –shock proteins: their relationships to the acquisition of thermotolerance in plants. Biotechnol. Appl. Biochem. 19, 41-49 (SCI 1.408).
  18. Jinn TL, Wu SH, *Yeh CH, Hsieh MH, Yeh YC, Chen YM & Lin CY (1993) Immunological kinship of class I low-molecular-mass heat shock proteins and thermostabilization of soluble proteins in vitro among plants. Plant Cell Physiol. 34, 1055-1062 (SCI 2.43).
  19. Tseng TS, Tzeng SS, Yeh KW, *Yeh CH, Chang FC, Chen YM & Lin CY (1993) Heat shock responses in rice seedlings: Isolation and expression of cDNA encoding class I low-molecular-weight heat-shock proteins. Plant Cell Physiol. 34, 165-168 (SCI 2.43).
  20. Tseng TS, Yeh KW, *Yeh CH, Chang FC, Chen YM & Lin CY (1992) Two rice (Oryza sativa) full length cDNA clones encoding low-molecular-weight heat shock proteins. Plant Molecular Biology 18: 963-965 (SCI 3.592).

CONFERENCES

  1. Hsu KH, Yeh CH, (2009) Physiological function assay of a heat- and abscisic acid-responsive rice gene, Os01g0719100. Annular Conference of Biomedical Sciences.
  2. Ke YT, Yeh CH, (2008) Characterization of rice group 3 LEA gene expression in development stages and stresses. The 6th International Symposium of Rice Functional Genomics.
  3. Chen MT, Lin, YP, Lin KF, Yeh CH, (2008) Regulation mechanism of Oshsp18.0 under treatment of L-azetidine-2-carboxylate and abiotic stresses. The 6th International Symposium of Rice Functional Genomics.
  4. Lin, YP, Chen MT, Lin KF, Yeh CH, (2008) Understanding the mechanism of HS-like response induced by denatured protein. Annular Conference of Biomedical Sciences
  5. Yeh CH, Lin, YP, Chen MT (2007) Characterization of a chemical responsive element on promoter of rice class I small heat shock protein genes. Annular Meeting of the American Society of Plant Physiologists.
  6. Lin YP, Chen MT , Yeh CH (2007) Characterization of an azatidine responsive element on promoter of rice class I small heat shock protein genes. Annular Conference of Biomedical Sciences.
  7. Yeh CH, Ting HM, and Ho TDH. Functional analysis of group 3 LEA proteins. 2006; Annular Meeting of the American Society of Plant Physiologists.
  8. Guan JC, You JW, Yeh CH,  Jinn TL, and Lin CY. A comprehensive analysis of rice small heat shock protein gene family. 2006; Annular Meeting of the American Society of Plant Physiologists.
  9. *Yeh CH, Wang , WK & (2004) Molecular evolution and expression patterns of the hemoglobin family in Arabidopsis. Annular Meeting of the American Society of Plant Physiologists.
  10. *Yeh CH, Travis D & Ho THD (2003) Expression of HVA1, a barley group III LEA protein, in Saccharomyces cerevisiae confers salt resistance. Annular Meeting of the American Society of Plant Physiologists.
  11. *Yeh CH, Chiu CC, Chen HI, Chen YM & Lin CY (2002) Physiological and Biochemical Analysis of Soybean (Glycine max) Small Heat Shock Proteins Induced by Azetidine-2-Carboxylic Acid. Annular Meeting of the American Society of Plant Physiologists.
  12. *Yeh CH, Chen YM & Lin CY (2001) Functional regions of rice (Oryza sativa) heat shock protein, Oshsp16.9, required for conferring thermotolerance in Escherichia coli. 2001 International Symposium on Plant Environment Interactions: Genes, Proteins, and Biotechnology.
  13. *Yeh CH, Chen YM & Lin CY (2001) Molecular analysis of the functional regions required for Oshsp16.9, a rice (Oryza sativa) class I low-molecular-mass heat shock protein, in relation to conferring thermotolerance. Annular Meeting of the American Society of Plant Physiologists.
  14. Young LS, *Yeh CH, Chen YM & Lin CY (1999) Molecular characterization of Oryza sativa 16.9 kDa heat shock protein. Annular Meeting of the American Society of Plant Physiologists.
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榮譽事項

  1. 1999 Dean of Science College Award, National Taiwan University

學會會員

  1. 中華植物學會
  2. 台灣中子學會
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實驗室成員

研究助理
吳青蓉
博士班學生
林冠甫 柯懿婷

碩士班學生
辜楚涵 連珮君
鄭榮儀



導生名單

年級 姓  名
大一 陳瑄敏 徐浩峰 林君儒 李柏寬  
大二 孔德馨 林仁豊 葉芸芷 黃昱齊  
大三 王又婷 黃畋勝 林漢莛    
大四 劉怡君 謝博閔 陳漢宇    

 

導生照片

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